Figure 1
From: High content screening identifies monensin as an EMT-selective cytotoxic compound
High content screen for EMT-selective compounds. (a) High content screen design. (b) Assay performance was assessed with a half-half plate using following parameters: the signal-to-noise (S/N) ratio = (µn − µp)/SDn, the signal-to-background (S/B) ratio = µn/µp, and the Z’ factor. (c) Results of the screen representing relative cell viability of TEM 4-18 mCherry (y axis) and PC-3E GFP (x axis) cells. Positions of selected compounds evaluated are indicated. (d–f) Cells cultured separately were treated for 72 h with serial dilution of salinomycin (n ≥ 5) (d), monensin (n ≥ 24) (e) or nigericin (n ≥ 5) (f). Relative cell viability was plotted against the logarithm of drug concentration. Data represent mean values ± SEM.
