Figure 2
From: High content screening identifies monensin as an EMT-selective cytotoxic compound
Monensin induces oxidative stress, apoptosis and a cell cycle arrest in TEM 4-18 cells. PC-3E and TEM 4-18 cells were treated with monensin (10 nM) for up to 72 h. Every 24 h, (a) the number of population doubling (n = 6), (b) cell cycle progression (PI incorporation) (n = 3), (c,d) apoptosis induction (C: Annexin V staining (n ≥ 3); D: PARP cleavage (n = 4)), (e) ROS formation (DHE probe oxidation) (n ≥ 7), and (f) clonogenic potential (n ≥ 12) were evaluated. Data represent mean values ± SEM.*p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001.
