Figure 7

Monensin sensitivity is correlated with increased cellular uptake in EMT-like cells. Cellular uptake of ³H-monensin in cells treated with ³H-monensin (10 nM) for (A,E) 6 h or (B) 1 h to 72 h. (C) Cells were treated with ³H-monensin (10 nM) for 6 h. Cellular monensin retention was measured 0, 30, 60, 90 and 120 min after the media was replaced. (D) Cells were incubated 6 h with ³H-monensin (10 nM) in presence of increasing concentration of cold monensin. Results were expressed in percentage of the radioactivity measured in cells treated with ³H-monensin only. Data represent mean values ± SEM of at least 3 independent experiments. *p < 0.05, **p < 0.01, ***p < 0.005, ****p < 0.0001. Different letters on top of bars indicate significant differences among time points (p < 0.05) (n ≥ 3).