Figure 1 | Scientific Reports

Figure 1

From: Ghrelin protects against contact dermatitis and psoriasiform skin inflammation by antagonizing TNF-α/NF-κB signaling pathways

Figure 1

Ghrelin expression is downregulated in OXA-induced contact dermatitis and IMQ-induced psoriasis mouse models, in both epidermis and dermis. (A) Ghrelin expression was evaluated by immunohistochemistry, and the results show that ghrelin levels are decreased in ear tissue after stimulation by OXA. (B) Ghrelin levels were diminished in OXA-induced contact dermatitis models, as shown by real-time PCR. (C,D) Total protein was extracted from skin tissue, and ghrelin was reduced upon stimulation with OXA, as shown by western blotting. (E) Expression of ghrelin was decreased in an IMQ-induced psoriasis mouse model, as assessed by immunohistochemistry. (F) Ghrelin expression was greatly decreased in mice stimulated with IMQ, as shown by real-time PCR. (G,H) Expression of ghrelin is downregulated at the protein level, as shown by western blotting. (I) Expression level of ghrelin in epidermis and dermis of dorsal skin in CTL and IMQ groups, as was detected by western blot. (J) Statistical analysis for relative protein level of ghrelin based on Western blot. (*p < 0.01 vs control group). (*p < 0.05, **p < 0.01, ***p < 0.005 vs the control group). Scale bar: 150 μm.

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