Figure 4
L-lactate and D-lactate production by lactobacilli after a 24 h incubation period with CaSki cells at 37 °C under 5% CO2. D-lactate and L-lactate concentrations after a 24 h incubation period at 37 °C under 5% CO2 in antibiotic-free cell culture medium were measured in duplicate within the same assays using Lactate Colorimetric kits. (A) D- and (C) L-lactate production by isolates grouped according to species are shown. Lines indicate medians, bars indicate the interquartile ranges and error bars indicate the ranges. (B,D) Lactate production by individual isolates is also shown with bars indicating the medians and error bars indicating the ranges of technical replicates within each assay. Mann-Whitney test was used for comparisons between species and p-values < 0.05 after adjustment for multiple comparisons were considered statistically significant. **Associations that remained statistically significant after adjustment for multiple comparisons. LA: Lactobacillus acidophilus probiotic (n = 2); LC: Lactobacillus crispatus (n = 7); LCR: Lactobacillus casei rhamnosus probiotic (n = 1); LG: Lactobacillus gasseri (n = 1); LM: Lactobacillus mucosae (n = 4); LV: Lactobacillus vaginalis (n = 6); LJ: Lactobacillus jensenii (n = 5).
