Table 2 Co-localization of MB and cell organelles. N is the number of the cells.

From: Fluorescence Polarization of Methylene Blue as a Quantitative Marker of Breast Cancer at the Cellular Level

Cell Line

0.05 mg/ml MB concentration

0.01 mg/ml MB concentration

Pearson’s R of MB and Nuclei

MDA-MB-231

0.43 ± 0.03 (N = 95)

0.44 ± 0.01 (N = 133)

MDA-MB-157

0.38 ± 0.03 (N = 112)

0.38 ± 0.02 (N = 97)

MCF-12A

0.36 ± 0.03 (N = 65)

0.41 ± 0.01 (N = 114)

MCF-10A

0.35 ± 0.03 (N = 88)

0.34 ± 0.02 (N = 100)

Pearson’s R of MB and Mitochondria

MDA-MB-231

0.35 ± 0.01 (N = 49)

0.39 ± 0.01 (N = 52)

MDA-MB-157

0.34 ± 0.01 (N = 60)

0.40 ± 0.01 (N = 57)

MCF-12A

0.23 ± 0.01 (N = 32)

0.20 ± 0.01 (N = 50)

MCF-10A

0.21 ± 0.01 (N = 42)

0.15 ± 0.01 (N = 46)

Pearson’s R of MB and Lysosomes

MDA-MB-231

0.29 ± 0.02 (N = 50)

0.34 ± 0.01 (N = 75)

MDA-MB-157

0.28 ± 0.02 (N = 59)

0.31 ± 0.01 (N = 67)

MCF-12A

0.22 ± 0.02 (N = 36)

0.20 ± 0.01 (N = 50)

MCF-10A

0.25 ± 0.02 (N = 54)

0.20 ± 0.01 (N = 57)