Figure 7
(a) Phospho-RTK arrays of HCB-514 were done at basal conditions (up) and upon EGF-stimulation (down). Each RTK is represented in duplicate in the arrays (two spots side-by-side), and three pairs of phospho-tyrosine positive controls are located in the corners of each array. (b) Western blot of EGFR phosphorylation, showing the different amount of phospho-EGFR and total EGFR compared to the SiHa cell line. Alpha-tubulin was used as endogenous control. (c) Quantification of phospho-EGFR normalized with total EGFR.
