Figure 4 | Scientific Reports

Figure 4

From: The discovery of Lake Hephaestus, the youngest athalassohaline deep-sea formation on Earth

Figure 4

Overview of high-resolution sampling, depth profile of magnesium and oxygen concentration and the RNA stratification through Lake Hephaestus interface. (a) Profiles of geochemical markers (Mg2+ and O2) through Lake Hephaestus and location of the established boundary for xerophilic cellular life (aw = 0.585). Positioning of the interface and brine was established by CTD profiling (Supplementary Fig. 1). The layers of the interface were collected for molecular and chemical analyses during two consecutive cruises and highlighted in grey (DEEPPRESSURE_2013) and yellow (SALINE_2014). Abbreviation used: UIF, upper interface; MIF, middle interface; LIF, low interface. Data points are mean ± standard error (n = 3). (b) Nucleic acids recovery from seawater, interface and brine. Types of mRNA gene transcripts are discussed through the text.

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