Figure 1
From: Trans-pairing between osteoclasts and osteoblasts shapes the cranial base during development
Localization of osteoclasts and osteoblasts in the basioccipital bone. (a) Representative micro-CT image of a mouse skull at P14 (n = 4). E, ethmoid; PS, presphenoid; BS, basisphenoid; BO, basioccipital (dorsal surface, green; parasagittal section, magenta); SOS, spheno-occipital synchondrosis; Arrows indicate the clivus. Voxel size, 40 μm. Scale bar, 5 mm. (b) Dorsal view of the cranial base shown in (a) and a scheme of the basioccipital bone. O, otic capsule; F, foramen magnum. Arrows indicate the clivus. Scale bar, 5 mm. (c) Safranin O staining of the cranial base in a representative section from a P3 mouse (n = 6). Arrows indicate the clivus of the basioccipital between the brainstem (pons and medulla, Po + Me) and longus capitis muscle (Mu). Pit, pituitary; Scale bar, 500 μm. (d) I2KI-contrast staining in a representative sample from a P16 mouse (n = 1). Arrows indicate the clivus. Voxel size, 10 μm. Scale bar, 500 μm. (e) Schematic showing the cranial base at an early postnatal stage. Asterisk, foramen magnum. BM, bone marrow. Red boxed region indicates area shown in (f). (f) Col1a1-AcGFP and MMP-9 immunostaining in representative sections from a P2 mouse (n = 2). (Left) Fluorescence detection of AcGFP (green), MMP-9 (magenta), and DAPI staining (white) in a P2 mouse. Scale bar, 100 μm. (Right) Magnified view of the boxed area in “DAPI”. Broken lines indicate endocranial and ectocranial cortical bone layers. Arrows, osteoclasts; arrowheads, osteoblasts; BM, bone marrow between cortical bones. A, anterior; P, posterior; D, dorsal; V, ventral. Maximum intensity projection images were acquired using a 63x objective and assembled from 3 slices at 2.86 μm intervals. Scale bar, 20 μm.
