Figure 4
From: Trans-pairing between osteoclasts and osteoblasts shapes the cranial base during development
Mice injected with anti-RANKL antibody. Mice were injected with saline or anti-RANKL antibody at P1 and then injected with alizarin complexone at P3. The basioccipital bone was then analysed at P4 (n = 4 each group). (a) MMP-9 immunostaining. (b) TRAP activity staining. (c) Merge image of MMP-9 and TRAP staining. (d) Alizarin labelling. (e) Collagen type I immunostaining. (f) Merged image of alizarin labelling and collagen type I staining. Bone resorption (arrows) and formation (arrowheads) surfaces are indicated. Maximum intensity projection images were acquired using a 40x objective and assembled from 12 slices at 0.6 μm intervals. Scale bars, 50 μm.
