Figure 1 | Scientific Reports

Figure 1

From: High sensitivity detection of extracellular vesicles immune-captured from urine by conventional flow cytometry

Figure 1

Characterization of PC3-derived EVs. (A) Nanoparticle tracking analysis (NTA). Average size and concentration were obtained in a Nanosight equipment capturing 3 videos of 60 s per measurement, with a focus −15 to +15 and camera level 12. ɸ: diameter. (B) Western Blot. EVs were loaded on SDS-PAGE and immunoblotted for β-actin (Sigma) and antibodies against tetraspanins [anti-CD9 (MEM62), -CD63 (MEM259) and -CD81 (MEM-38)]. Three gels were loaded: one gel, under non-reducing conditions with 2.2·109 particles, for CD9 and CD81 detection, exposed for 2 min; a second non-reducing gel with 6.8 · 109 particles, for CD63 detection, exposed for 1 h; and the third gel under reducing conditions, for actin detection, exposed for 40 s. The experiment shown is representative of 4.

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