Figure 5

Measurement of siderophore production using chrome azurol sulfonate (CAS) assay for Xag strains. The halo from Xag strains were observed (A) under iron-rich conditions on a XVM2-CAS plate or (B) under iron-deficient conditions on a XVM2-CAS-BP plate. (C) Sizes of colonies (white) and siderophore halo zones (black) among Xag strains were measured at 3 days after incubation. Error bars represent the mean of three biological replicates with standard deviations. Different letters represent statistical difference using the least significant difference test, P ≤ 0.05. All experiments were repeated at least three times with three biological replicates.