Figure 6
From: Targeted editing of the PSIP1 gene encoding LEDGF/p75 protects cells against HIV infection
LEDGF depletion results in an increase of the silent HIV reservoir. (a) Schematic representation of the dual-colored VSV-G pseudotyped HIVOGH reporter virus carrying an eGFP cDNA driven by the viral LTR promoter in the nef position and an entire constitutive transcriptional unit (EF1a-mKO2) inserted downstream. (b) HIV provirus integration is greatly diminished in the absence of LEDGF/p75 when compared to SupT1_WT cells, as evidenced by the lower %mKO2 positive cells for a given virus concentration. Error bars represent SD. Student’s t test was performed using GraphPad Prism 7.0 software. Sample means were considered significantly different from the WT control at p < 0.05 (*). (c) The latent fraction for the respective cell lines for different virus concentrations. LEDGF KO increases the fraction of silently infected cells ((% eGFP−, mKO2+ cells)/% mKO2+ cells) ∗ 100. eGFP, Enhanced Green Fluorescent Protein; mKO2, Mutant Kusubira Orange 2. The experiment was performed three times. The plots are representatives of one of three independent infection experiments.
