Figure 8
From: Targeted editing of the PSIP1 gene encoding LEDGF/p75 protects cells against HIV infection
HIV replication is hampered in SupT1_LEDGF D366N cell line as compared to the SupT1 WT cell line. (a) HIV-1 replication assay. The cell lines were challenged with the laboratory HIVNL4.3 strain at a final concentration of 5.0*102 pg p24. Replication was monitored by daily sampling of the p24 levels in the cell culture supernatant. (b) Introduction of the D366N mutation in LEDGF/p75 renders SupT1 cells refractory to transduction as shown by a 4-fold decrease in %mKO2 expressing cells for a given VSV-G pseudotyped HIVOGH virus concentration. Student’s t test was performed using GraphPad Prism 7.0 software. Sample means were considered significantly different from the WT control at p < 0.05 (*). (c) In addition to the lower transduction efficiency observed for SupT1_LEDGF D366N, the latent fraction is greater when compared to SupT1_WT cells, even at higher concentrations of HIVOGH virus. eGFP, Enhanced Green Fluorescent Protein; mKO2, mutant Kusubira Orange 2. The plots represent one of three independent infection experiments.
