Figure 9
From: Targeted editing of the PSIP1 gene encoding LEDGF/p75 protects cells against HIV infection
HIV replication remains hampered in SupT1 LEDGF D366N cell line at high MOI as compared to the SupT1 WT and SupT1 LEDGF KO cell line. (a) HIV-1 replication assay at low MOI. The cell lines were challenged with the laboratory HIVNL4.3 strain at a concentration of 1.5*106 pg p24 for 2 h. The cells were then washed to remove the virus to result in 5.0*102 pg/ml p24 final concentration and monitored for replication. (b) HIV-1 replication assay at high MOI. The cell lines were challenged with the laboratory HIVNL4.3 strain at a final concentration of 1.5*106 pg p24. Replication was monitored by daily sampling of the p24 levels in the cell culture supernatant. Each plot represents one of three independent infection experiments.
