Figure 7

Adjuvant effect of mmCT and dmLT is also dependent on THBS1 and ITGB1. Purified CD14⁺ monocytes were incubated with siRNAs specific for THSB1 (A,B), ITGB1 (C,D), All-Star Control (E,F), and GNAS (G,H) for 24 h. Cells were then left untreated or were treated for 16 h with mmCT or dmLT, washed, and co-cultured for 3 days with autologous CD4⁺ T cells plus SEB. Bars represent mean plus SEM of IL-17A levels in culture supernatants measured by ELISA.