Table 2 Testing of commercial available enzymes for presence of plasmid sequences. The first test (PCR reaction w/o template) was run without any template. The mastermix contained all necessary additives as well as specific primers. A minus sign “−” denotes a negative result, whereas a plus sign “+” shows a positive result. Enzymes tested negative in the first experiment were not tested in the second experiment and results are therefore not available (N/A). If an enzyme was tested positive in the first run, 0.25 µl of the polymerase was added as template to a mastermix tested previously negative. If the results were positive the enzyme added as template contained plasmid residues.

Polymerases	Plasmid Targets
	Ori		Bla_Tem-1		CmR
	PCR reaction w/o Template	Enzyme Preparation as Template	PCR reaction w/o Template	Enzyme Preparation as Template	PCR reaction w/o Template	Enzyme Preparation as Template
^aHotStarTaq DNA Polymerase	+	+	+	+	−	N/A
^biTaq DNA Polymerase	−	−	−	−	−	N/A
^cTaq DNA Polymerase	−	N/A	−	N/A	−	N/A
^dGoTaq G2 Hot Start Polymerase	−	N/A	−	N/A	−	N/A
^cPhusion High-Fidelity DNA Polymerase	−	N/A	−	N/A	−	N/A
^biProof High-Fidelity DNA Polymerase	−	N/A	−	N/A	−	N/A
^cLuna Universal qPCR Master Mix	−	N/A	−	N/A	−	N/A
^cLuna Universal Probe qPCR Master Mix	−	N/A	−	N/A	−	N/A
^cLuna Universal Probe One-Step RT-qPCR Kit	−	N/A	−	N/A	−	N/A
^dGoTaq Probe qPCR Master Mix	−	N/A	−	N/A	−	N/A
^eEvaGreen 2X qPCR Express Mix-ROX®	+	+	+	−	−	N/A
^biTaq Universal Probes Supermix	+	−	+	−	−	N/A
^fTaqMan Universal PCR Master Mix	−	N/A	−	N/A	−	N/A

^aEnzymes sold by Qiagen; ^bEnyzmes sold by BioRad; ^cEnzymes sold by New England Biotechnologies; ^dEnyzmes sold by Promega, ^eEnzymes sold by ABM Life Sciences; ^fEnzymes sold by Thermofisher.

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