Figure 5

Estimation of microbial attributes. (A) Proportion of reads associated with trophic lifestyle (black hyphen), normalized to total abundance of reads annotated for COG categories with variable relative abundance depending on trophic life style (copiotrophic and oligotrophic) as percentage, as determined by comparison of 34 genomes of marine bacteria by Lauro et al. (2009). Grey horizontal lines represent the mean proportion of reads associated with copiotrophic life style within the selected COG in oligotrophic organisms (lower line, ratio copio:oligo functions from 50:50) and copiotropic organisms (upper line, ratio copio:oligo functions from 75:25). All samples with less than 100 hits within the selected COG categories were removed from the analysis. The detailed relative abundance of each COG functions investigated is available in Table S2. (B) Relative abundance of reads associated with microbial attributes; i.e., reads aligned against a genome obtained from an organism with described features (endospore formation, type of habitat, motility, oxygen requirement, temperature range) obtained from NCBI website and referenced in MEGAN. Read abundance was normalized to total abundance of reads annotated for each microbial attribute, as percentage.