Figure 1

Rearing conditions and opsin gene expression of newly hatched medaka. (A) White LED shows two peaks at 451 and 581 nm. Green LED shows one peak at 518 nm. (B) Experimental schedule. White and green LED irradiations started from just after hatching (0 dph) to 60 dph. Fish were sampled at 60 dph for gonadal histology and genotyping. Progeny tests were conducted at 90 dph by artificial insemination. (C) RT-PCR analysis of 8 opsin genes and ef-1a in the eyes of 3 dph and adult medaka. The λmax values for each opsin protein are from Matsumoto et al. 200630.