Figure 3
Progeny test of sperm obtained from sex-reversed males. (A) A sex-reversed male showing a female genotype (dmy−/−), female-specific white body color, and male-specific secondary sexual characteristics in fins (a parallelogram dorsal fin and a deeply cut anal fin, arrows). (B) Spermatozoa obtained from a sex-reversed male (left) were morphologically normal and indistinguishable from those of a normal male (right). (C) Density of spermatozoa of normal males (n = 3) and a sex-reversed male (n = 2). P < 0.05. (D) Genomic DNA PCR of the sperm (Sp) and a fin (Fin) of a sex-reversed male using a primer set for dmy/dmrt1 genes. (E) Genomic DNA PCR for F1 offspring obtained from sex-reversed males (n = 2) (lanes 1–9) using a primer set for dmy/dmrt1 genes. Fin clips of adult males and females were used for positive controls. DW, distilled water for negative control; MW, molecular weight marker. (F) Histological observation of a gonad of F1 offspring. Dotted line shows an ovary. Scale bars = 2 mm (A), 10 mm (B), 0.5 mm (F).
