Table 1 In vivo properties of the yFPs.

FP	Color^a	Exc. max^b	Em. max^c	In vivo bright-ness ± SD^d	Photo-stability^e	T_half 1^f	T_half 2^f	Daily var.^g	Life-time^h	Monomerism ± SDⁱ	pKa^j	Hill coeff.^k
ymTq2	CFP	439	475	2.24	0.94 ± 0.03	490		0.30	4	0.20 ± 0.42	4.48	0.64
ymNeongreen	GFP	506	517	4.16	0.98 ± 0.01	0.19	0.001	0.03	3.2	0.15 ± 0.60	5.42	1.08
ymNeongreen	YFP	506	517	2.48	0.95 ± 0.02	5752	34	0.05	3.2	0.15 ± 0.60	5.42	1.08
ymVenus	YFP	515	527	1.90	0.62 ± 0.03	32	0.40	0.14	2.9	0.49 ± 0.81	5.50	0.62
ymYPET	YFP	516	526	1.72	0.64 ± 0.04	46	0.14	0.13	3	0.12 ± 0.40	4.89	0.91
ytdTomato	RFP	552	580	1.01	0.95 ± 0.01	1460		0.07	n.d.	0.30 ± 0.67	4.76	1.14
ymScarletI	RFP	568	591	1.02	0.74 ± 0.07	118		0.12	n.d.	0.15 ± 0.42	4.26	0.44

^aSpectral color class. ^bExcitation maximum. ^cEmission maximum. ^dBrightness in budding yeast normalized to either mCherry (for CFPs and GFPs) or mTq2 (for YFPs and RFPs). ^eFluorescent fraction remaining at the last timeframe, normalized to the first frame. ^fT-half times in seconds obtained via a one-phase or two-phase exponential decay fit. ^gCoefficient of variation (CV) of the mean brightness of 3 days as an indication of day-to-day variation. ^hFluorescence lifetime in nanoseconds determined by frequency domain. ⁱMonomerism depicted by the mean amount of OSER structures per cell. ^jpH value giving 50% decrease in fluorescence. ^kHill coefficient of the pH stability. Abbreviations: SD; standard deviation, n.d.; not determined.

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