Figure 3

Proteomic profiling of distinct macrophage subsets from different phases in APAP-induced liver injury. (A) Macrophage populations during distinct phases from 8–10 mice were used and pooled to acquire proteins for mass spectrometry analysis. Venn diagram of the quantified proteins in 24 h Ly6C^hiCX₃CR1^lo macrophages and 72 h Ly6C^loCX₃CR1^hi macrophages. (B) Volcano plot generated by differential analysis of the proteome profiles of 72 h Ly6C^loCX₃CR1^hi macrophages compared with those of 24 h Ly6C^hiCX₃CR1^lo macrophages. Significantly up-regulated proteins were shown as red dots and down-regulated proteins were shown as green dots. The proteins with fold change ≥2 and p < 0.05 were considered to be significant differentially expressed proteins. (C) Gene Ontology (GO) enrichment analysis of differentially expressed proteins was classified by their biological functions and arranged according to their statistical significance (-log₁₀ p value on x axis) from DAVID. Biological processes involved in immune response were shown. (D) Validation of the indicated differentially expressed genes by qPCR, presented relative to Gapdh. Data shown are representative of three independent experiments (n = 3/group). Results represent mean ± SEM (*p < 0.05, **p < 0.01, ***p < 0.001).