Figure 4
From: Green tea extract promotes DNA repair in a yeast model
GTE promotes the HR activity. (A) Schematic of the MAT locus, HML, and HMR used for the single-end invasion (SEI) assay. The MAT locus, HML, and HMR share identical W, X and Z1 sequences. Galactose-induction of pGAL-HO endonuclease initiates the formation of a double-strand break at the HO cutting site. Repair of the double-strand break may occur via strand invasion by the HMRa region, which can be detected using qPCR with primers for SEI-F and SEI-R. (B) GTE enhanced HR repair at DSB sites, as measured by the SEI assay. White bars represent the control group and gray bars represent the GTE-treated group. Data are presented as means ± S.D.
