Figure 2
From: Early overnutrition reduces Pdx1 expression and induces β cell failure in Swiss Webster mice
Glucose and insulin tolerance in adults. Body weight in males (a) to postnatal day (P) 71 (n = 15–16) and females (b) to P171 (in females, main effect of group with early overnutrition > control by two-way repeated measures ANOVA, p < 0.05; n = 7–8). Plasma leptin levels (c) and plasma triglyceride levels (d) at P50 in males, and diabetes incidence (e) in males to 52 weeks of age (p = 0.021 by log-rank test). Blood glucose response (f), glucose AUC (g), plasma insulin response (h), and insulin AUC (i) after intraperitoneal injection of 2 g/kg of glucose in 6 hr fasted male mice at P58. Blood glucose response normalized to baseline (j) and glucose AUC (k) in response to intraperitoneal injection of 0.75 U/kg insulin (Novolin) in 4 hr fasted male mice at P85. Data in (a,b,f,h and j) represent median ± interquartile range. Box and whisker plots (c,d,g,i,k) represent the interquartile range (box) and minimum to maximum values (whiskers), with line at the median. AUC: area under the curve with baseline = 0. Analyses by two-way repeated measures ANOVA with Bonferroni post-hoc (a,b) or unpaired t-test (c); *p < 0.05, **p < 0.005.
