Figure 3
From: Early overnutrition reduces Pdx1 expression and induces β cell failure in Swiss Webster mice
Islets in non-diabetic adult mice. β cell mass normalized to body weight (a), number of islets normalized to pancreas area (b), and islet size distribution (c) measured by insulin-positive area per islet, in non-diabetic early overnutrition vs control males at postnatal day (P) 100. Insulin secretion (d) of 80 size-matched isolated islets per mouse perifused with glucose (3 and 20 mM) and KCl (30 mM) at P80. Insulin content per islet (e) averaged from 5 size-matched islets per mouse at P94, and total pancreas insulin content (f) at P88. Expression of genes important for β cell function (g) and genes implicated in β cell lipotoxicity (Srebp1c, Ucp2, Plin2), endoplasmic reticulum stress (Ddit3, Plin2), and oxidative stress (Nos2) (h) in islets isolated at P50. Box and whisker plots (a–c,f-h) represent the interquartile range (box) and minimum to maximum values (whiskers), with line at the median. Line graph (d) represents median ± interquartile range. Floating bars (e) represent the range of values with line at the median. Analyses by unpaired t-tests (for a,b,e), or unpaired t-test with Bonferroni correction for multiple comparisons (g); *p < 0.05.
