Figure 5
From: Identifying Windows of Susceptibility by Temporal Gene Analysis
Genes down-regulated by ZIKV infection of cortical progenitors reveal a SWOT clock similar to microcephaly. Expression data for ZIKV infected cortical progenitors were re-analyzed using more stringent criteria to establish significantly changing genes and SWOT Clocks of the (A) all genes (left), down-regulated (middle) and up-regulated genes (right) were generated. The down-regulated SWOT Clock showed a similar profile to the Microcephaly clock. (B) Intersection of Microcephaly genes with the ZIKV genes filtered through the developmental time-course data (left). To examine a broader range of genes potentially contributing to the microcephaly phenotype the 1st degree neighbors of the intersection SWOT clock were retrieved from STRINGdb then re-filtered through the time-course data and ZIKV data, then the process was repeated again to generate an expanded network (right). The Legend: Pluripotency (PP); Neuroectoderm (NE), Neural Differentiation (ND), Cortical Specification (CS), Deep Layers (DL), Upper Layers (UL). (C) Network analysis of Intersection genes shows distinct three communities contribute to the gene intersection. Significant enrichment or depletion is based on a FDR adjusted p-value < 0.1.
