Figure 4
Expression of IL-1β receptors. Adult and fetal tenocytes express significantly more IL1R1 and less IL1R2 and IL1RN than ESC-tenocytes when cultured in 2D in (A) the absence of IL-1β, (B) the presence of IL-1β and (C) 3D culture. The relative expression compared to the housekeeping gene (18 s) is shown. Error bars represent the s.e.m. of three independent biological replicates. *p < 0.05 using a single factor ANOVA with post hoc Tukey test. (D) Immunocytochemistry demonstrates the presence of IL1R1 protein (red) in the fetal and adult tenocytes but not in the ESC-tenocytes. DAPI staining of the nuclei is shown in blue. Scale bar = 20 µm.
