Table 1 Primer sequences used in quantitative PCR.

From: A novel mechanism for the protection of embryonic stem cell derived tenocytes from inflammatory cytokine interleukin 1 beta

Gene

Forward primer

Reverse primer

18S rRNA

CCCAGTGAGAATGCCCTCTA

TGGCTGAGCAAGGTGTTATG

MMP1

CTTTGATGGACCTGGAGGAA

GAATGGCCAAATTCATGAGC

MMP2

CAGGAGGAGAAGGCTGTGTT

AGGGTGCTGGCTGAGTAGAC

MMP3

TGGACCTGGAAAAGTTTTGG

GACCAAGTTCATGAGCAGCA

MMP8

TTTGATGGACCCAATGGAAT

TTCATGGGCAGCAACAATAA

MMP9

GAGATCGGGAATCATCTCCA

CCAAGAGTCGCCAGTACCTC

MMP13

GCCACTTTGTGCTTCCTGAT

CGCATTTGTCTGGTGTTTTG

SCX

CCCAAACAGATCTGCACCTT

ATCCGCCTCTAACTCCGAAT

TNMD

GTCCCTCAAGTGAAGGTGGA

CCTCGACGGCAGTAAATACAA

TNC

AACCCGTCCAAAGAGACCTT

GCGTGGGATGGAAGTATCAT

COL1A1

TGCGAAGACACCAAGAACTG

GACTCCTGTGGTTTGGTCGT

COMP

AGAACATCATCTGGGCCAAC

CGCTGGATCTCGTAGTCCTC

THBS4

GGGAAATGGGGTTACCTGTT

CGGGTAGCAGGGATGATATT

SOX9

GCTCTGGAGACTTCTGAACGA

GTAATCCGGGTGGTCCTTCT

IL1R1

GAAGCGCATAAAGGGCACTA

CGTGGGCCTGATTTCATCTA

IL1R2

TCTGGCACCTACATCTGCAC

CAGGGCAGCTTCTGTCTTCT

IL1RN

GCCTGTGTCAAGTCTGGTGA

CCTCCTTGTTCTTGCTCAGG

Back to article page