Figure 4
Cytoprotective effects of 5-ASA and AZTP (A) DCFDA based fluorescence assay for the measurement of intracellular ROS upon treatment with 5-ASA (5 mM) or AZTP(10 μM) in the presence or absence of H2O2 (100 μM, 1 h). n = 3; p value (≤0.05), *vs H2O2. Kruskal-Wallis Test with Dunnet post hoc analysis was performed. (B) Senescence associated β-galactosidase activity in HCEC-1CT. Positive (blue) cells were counted under inverted microscope (100x). Positive cells counted per field of view (FOV). 5 FOV were counted per sample. Experiment was performed in biological duplicates. (C) Model depicting molecular effects of 5-ASA (mesalamine) and AZTP (azathioprine) in mucosal healing.
