Figure 2 | Scientific Reports

Figure 2

From: Nuclear import of Xenopus egg extract components into cultured cells for reprogramming purposes: a case study on goldfish fin cells

Figure 2

Digitonin-induced permeabilization of adherent fin cells in relation to cell density. Cells were permeabilized by digitonin at 7.5 µg/mL (Dig7.5) and 30 µg/mL (Dig30). After 2 min exposure at 4 °C, cells were labelled with Hoechst 33243 (upper picture) and the non membrane permeant propidium iodide (PI) (lower pictures). The permeabilized cells were identified by a red PI fluorescence observed in low and high density areas. Note that no red fluorescence was detected in non-permeabilized cells (Control cells) included in this experiment. These pictures are representative of seven independent replicates. Scale bar = 20 µm.

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