Figure 3 | Scientific Reports

Figure 3

From: Nuclear import of Xenopus egg extract components into cultured cells for reprogramming purposes: a case study on goldfish fin cells

Figure 3

Cell morphology (A) and loss of cytoplasmic proteins (B) after digitonin-induced permeabilization. The cells were permeabilized for 2 min with 30 µg/mL digitonin at 4 °C. (A) The morphology of permeabilized (Dig30) and non-permeabilized (Control) cells was observed by phase-contrast microscopy. Note the nuclear envelope that was more contrasted (arrow) in permeabilized cells than in control cells. Scale bar = 20 µm. (B) Digitonin-permeabilized (Dig30) and non-permeabilized (Control) cells were lysed with RIPA buffer added to the adherent cells. Protein lysates were submitted to SDS-PAGE then silver stained. The loss of a protein band at about 60 kDa was observed (arrow) in the permeabilized cells.

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