Table 2 HDX analysis results of Nm23-H1.

From: deMix: Decoding Deuterated Distributions from Heterogeneous Protein States via HDX-MS

m/z

rt (m)

peptide

sites

D2O labeling times (Native)

D2O labeling times (Oxidative)

30 s

1 m

3 m

5 m

10 m

30 m

60 m

30 s

1 m

3 m

5 m

10 m

30 m

60 m

420.69 (2+)

5.96

ANCERTF

2~8

1

1

1

1

1

1~2

2~1

1

1

1

1

1

2

2

566.99 (3+)

8.71

FIAIKPDGVQRGLVGE

8~23

1

1

1

1

1

1

2~0

1~3

2~0

1~3

1~4

3~1

3

4

776.45 (2+)

8.01

IAIKPDGVQRGLVGE

9~23

1

1

1

1

1

1

1

2

2

1~4

1~4

4~1

2~5

5~2

517.97 (3+)

8.01

IAIKPDGVQRGLVGE

9~23

1

2~0

1

1~3

1

2~0

1

1~4

1~4

1~4

1~4

4~1

4

4

663.64 (4+)

10.55

IAIKPDGVQRGLVGEIIKRFEQKG

9~32

0~2

0~3

2

0~3

0~3

2~0

2

2

3~0

1~4

1~4

1~4

2~5

4

767.70 (4+)

11.96

IAIKPDGVQRGLVGEIIKRFEQKGFRL

9~35

×

1

1

1

1~3

×

1

3~0

4~0

1~5

1~5

1~5

2~6

3~8

512.31 (3+)

8.01

IIKRFEQKGFRL

24~35

0~2

1

1

1

1

0~2

1

1~0

1

1

1

1

2~0

1~3

490.31 (2+)

9.84

FRLVGLKF

33~40

0~1

0~1

0~1

1~0

1~0

0~1

1~0

0~1

1~0

1~0

1~0

1

1

1~3

941.48 (3+)

10.2

MQASEDLLKEHYVDLKDRPFFAGL

41~64

7

8

9

9

10

11

11

10

10

10

11

11

11

11

569.81 (4+)

10.2

DLLKEHYVDLKDRPFFAGL

46~64

6

6

7

7

8

8

9~8

8

8

8

8

9

9

9

602.99 (3+)

9.14

LKEHYVDLKDRPFF

48~61

3

3

4

4

4

4

4

4

4

4

4

4

5

4

452.49 (4+)

9.14

LKEHYVDLKDRPFF

48~61

3

3

4

4

4

4

4

4

4

4

4

4

4

4

1024.55 (2+)

9.49

LKEHYVDLKDRPFFAGL

48~64

5

5

6

6

6

6

6

6

6

6

7

6

7

7

559.96 (3+)

10.13

HYVDLKDRPFFAGL

51~64

5

6

6

6

6

7

6~7

6

6

6

7

7

7

7

650.86 (2+)

7.3

LVKYMHSGPVVA

64~75

1

1

1

1~2

2~1

2

2

2~1

2~1

2~1

2

2

2

2

716.38 (2+)

8.08

LVKYMHSGPVVAM

64~76

1

1

1

1

2

2

2

2~1

2

2~1

2

2

2

2

594.32 (2+)

6.59

VKYMHSGPVVA

65~75

2~0

2~0

2~0

2~0

2

2

2

2~0

2~0

2

2

2

2

2

680.85 (2+)

8.08

PADSKPGTIRGDF

96~108

2

2

2

2

3

3

3

3

3

3

3

3

3

3

852.10 (3+)

10.55

CIQVGRNIIHGSDSVESAEKEIGL

109~132

5

5

5

5

6

7

7

7

8

8

9

10

11

12

586.28 (2+)

9.77

WFHPEELVD

133~141

1~3

1~2

1~2

1~2

1~2

2

2

2

2

2

2

2

3

3

  1. Each cell represents the deuteration numbers of input peptides under each condition, where ‘x’ means that deuterated distribution is not detected and #~# means that a peptide is observed as two deuterated forms (more abundant form precedes). “rt (m)” column shows a retention time of a peptide in minutes and “sites” column represents the start and end positions of a peptide in Nm23-H1.
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