Figure 1
MTT assay of NRK52E- and RMC cells affected by testosterone, bicalutamide, and R1881 in 25 mM hyperglycemic medium. NRK52E cell line (1 × 104 cells/well) on 24-well plate was incubated in medium containing 5% bovine serum and 0.4% PBS overnight until adhered. Finally, the medium was replaced fresh with serum free medium. RMC cell line (1 × 104 cells/well) on 24-well plate was incubated in medium containing 25 mM glucose, 15% FBS, 0.4% PBS, and 0.8% G418 overnight until adhered. Finally, the medium was replaced fresh with 2% FBS. At final step, the two cells were simultaneously treated as indicated. (a) NRK52E + testosterone (1~103 nM), (b) NRK52E + bicalutamide (3.75~60 μM), (c) RMC + testosterone (1~103 nM), (d) RMC + R1881 (1~103 nM), and (e) RMC + bicalutamide, and (f) RMC + bicalutamide + testosterone for duration as indicated. Experiment was performed in triplicate
