Figure 4

Late apoptosis of RMC cell lines affected by treatment of bicalutamide. (a) The apoptosis profile affected by treating with bicalutamide respectively at 24 and 48 h when examined with Annexin V and 7-ADD staining. (b) The quantified cell population in each quadrant of (a) at 24 h. (c) The quantified cell population in each quadrant of (a) at 48 h. (d) The representative protein expression of autophagic marker LC3B-I and LC3B-II after Bic treatment for 48 h. Late apoptosis of RMC cell lines affected by bicalutamide. Experiment was performed in triplicate (n = 3).