Figure 1
From: Activation of the PERK-ATF4 pathway promotes chemo-resistance in colon cancer cells
Activation of the unfolded protein response pathways in colon cancer cells. (A) Western blot showing the expression of the PERK pathway genes in six colon cancer cells. GAPDH was used as loading control. (B) Western blot showing the expression of the IRE1 pathway genes in cells in (A). Alpha Tubulin was used as loading control. (C) Luciferase reporter assay showing the transcriptional activity of ATF6 in the cells in (A). Activity of ATF6 was normalized to Renilla luciferase. (D) Realtime RT-PCR showing the relative expression level of IRE1, PERK and BiP mRNA in six colon cancer cells. GAPDH was used as loading control. Data are represented as mean ± SEM or the mean alone.
