Figure 3
From: HtrA4 may play a major role in inhibiting endothelial repair in pregnancy complication preeclampsia
Validation of EPC clones. (a) RT-PCR confirmation of gene markers. CD144 and PECAM1 are positive markers of EPC, whereas CD45 is a negative marker of EPCs but a positive marker of monocytes. Full length gels are presented in Supplementary Fig. 2. (b) Immunofluorescent staining of Dil-labelled AcLDL (red) in EPCs. Human Ishikawa cells were used as a non-EPC control line, which did not uptake AcLDL. Nuclei were stained with DAPI (blue). Representative images at 10x magnification are shown. (c) Confirmation that EPCs form endothelial tubes. All EPCs formed tube-like structures on Matrigel. A representative image at 4x magnification is shown.
