Figure 2
Placement of the MAGs into their phylogenetic context. Taxonomy of the MAGs (metagenome-assembled genomes) was refined by placing them into a phylogenetic tree using PhyloSift v. 1.0.1 with its updated markers database for the alignment and RAxML v. 8.2.10 on the CIPRES web server for the tree inference. This tree includes the 36 MAGs (red dots), all taxa previously identified by Burgess et al. (2012) with complete genomes available on NCBI (n = 148)82, and 3,102 archaeal (yellow) and bacterial (grey) genomes previously used in Hug et al. (2016)79,80,81. The complete tree in Newick format and its alignment of 37 concatenated marker genes can be found on Figshare75,100. Branches with MAGs found in Arkashin Schurf (ARK) and Zavarzin Spring (ZAV) are enlarged (orange nodes). Blue: taxa from Burgess et al. (2012), black: taxa from Hug et al. (2016). GCA IDs from NCBI are shown for the closest neighbours of the MAGs. (a) Microbial tree of life, reconstructed with genomes representing taxa reported in Burgess et al. highlighting the placement of MAGs in this study; (b) Dictyoglomales, Thermoanaerobacteriales, Caldisericales, and Mesoaciditogales; (c) Nitrosphaera, Bathyarchaeota, Korarchaeota, and Crenarchaeota; (d) Chloroflexales; (e) Euryarchaeota; and (f) Deferribacteriales, Desulfobacteriales, and Aquificales. ARK-02, ARK-03, ARK-04, ZAV-04, ZAV-08, ZAV-09, and ZAV-15 can be found in Supplementary Figure S1.
