Figure 4

Pangenomic comparison of shared genera between pools. Desulfurella genera (a) and Sulfurihydrogenibium genera (b) identified in both Arkashin Schurf (ARK) and Zavarzin Spring (ZAV) are visualized respectively in anvi’o against reference genomes downloaded from NCBI. ARK-08 and ZAV-10 were compared to three representative Desulfurella genomes including D. multipotens (GCA_900101285.1), D. acetivorans (GCA_000517565.1) and D. amilsii (GCA_002119425.1), while bins ARK-13 and ZAV-16 were compared to four representative Sulfurihydrogenibium genomes including S. subterraneum (GCA_000619805.1), S. yellowstonense (GCA_000173615.1), S. azorense (GCA_000021545.1) and S. sp. YO3AOP1 (GCA_000020325.1). Genomes are arranged based on a phylogenetic tree of shared single-copy core genes produced in anvi’o using FastTree v. 2.1. Gene clusters have been grouped into categories based on presence/absence including: ‘Single-copy core genes’ (gene clusters representing # genes from Campbell et al.)64, ‘Universally shared’ (gene clusters present in all genomes), ‘Often Shared’ (gene clusters present in two or more genomes) and ‘Unique’ (gene clusters present in only one genome). Gene calls were annotated in anvi’o using NCBI’s Clusters of Orthologous Groups (COG’s). Gene clusters with an assigned NCBI COG are indicated in black.