Figure 6
Small RNA hydrolysis mechanism of CpsORN. (A) Structural comparison of unliganded CpsORN and separated uridine-bound CpsORN shows that the α8-α9 loop region undergoes movement upon ligand binding. (B) The relative enzyme activities of the mutants are calculated by taking the activity of wild-type CpsORN as 100%. Experiments were performed in triplicate, and the error bar indicates the standard deviation. (C) CD spectra were recorded for wild-type and mutants in the range of 190–250 nm at 20 °C.
