Table 3 Seismic mimic experiment at Kopanang. After closure of the borehole for 30 min. the flow was released suddenly and sampled for chemical composition and HPC every 20 min. for 80 minutes. BDL: below detection limit. mg/L unless otherwise stated. Number of biofilm pieces reported are those collected in the 20 minute time frame.

	+20 min	+40 min	+60 min	+80 min
Volume(L)	101	80	72	63
T(°C)	31.3	31.3	32.0	32.4
pH	8.15	8.10	8.17	8.16
EC (mS/m)	47.9	49.1	49.1	49.1
TDS (ppm)	370	378	374	366
O₂d	23	bdl	bdl	bdl
TOC	2.54	1.88	1.23	1.93
DOC	0.9	0.7	0.5	0.7
Al³⁺	0.054	0.041	0.029	0.039
Ba²⁺	0.040	0.023	0.032	0.028
Ca²⁺	47.9	49.1	49.1	49.1
Cd²⁺	<0.001	<0.001	<0.001	<0.001
CL⁻	16.5	15.9	15.7	15.6
Cr²⁺	<0.006	<0.006	<0.006	<0.006
Cu³⁺	0.006	0.004	0.003	0.006
Fe	0.005	0.004	0.000	0.012
Li	0.011	0.015	0.014	0.013
Mg²⁺	0.76	0.82	0.74	0.74
Mn²⁺	0.015	0.007	0.010	0.010
Mo²⁺	<0.003	<0.003	<0.003	<0.003
Na⁺	111.3	120.35	116.95	110.91
Ni²⁺	<0.010	<0.010	<0.010	<0.010
K⁺	0.74	1.11	0.84	0.61
Pb	<0.010	<0.010	<0.010	<0.010
Si⁴⁺	8.717	8.555	8.616	8.707
Sr²⁺	0.046	0.024	0.033	0.037
V³⁺	<0.006	<0.006	<0.006	<0.006
Zn²⁺	0.023	0.014	0.012	0.011
F	0.25	0.24	0.23	0.24
Cl	16.5	15.9	15.7	15.6
NO₂₋	bdl	bdl	bdl	bdl
Br⁻	0.0468	0.0639	0.061	0.086
NO₃₋	0.0482	−0.05	−0.05	−0.05
PO₄³⁻	bdl	bdl	bdl	bdl
SO₄²⁻	29.7	30.045	29.865	29.288
NH₄⁺	0.12	0.18	0.06	0.17
Tot hard	9.96	11.24	10.04	9.80
HPC	6	>1000	0	0
# biofilm pieces	30	138	25	32

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