Figure 3

(a) IEF of PASylated Adnectin C and native protein on 7 cm IPG strips. Both proteins have similar net charges at pI = 6.5–6.8. Lane 1 is a marker, lane 2 is Adnectin C-PAS#1(200) and lane 3 is Adnectin C. Zeta potential distribution curves of: (b) Adnectin C; (c) Adnectin C-PAS#1(200). Measurements showed a negligible difference in zeta potential between Adnectin C-PAS#1(200) (−5.28 mV) and the native protein (−6.15 mV).