Figure 2
MBV decrease pro-inflammatory cytokine secretion from glia and neuroprotect RGCs. (a–c) In unprimed microglia, LPS/IFNγ increased IL-1β, IL-6, and TNF-α secretion. In LPS/IFNγ primed microglia, IL-1β, IL-6, and TNF-α secretion increased in media, but both UBM-ECM (250 µg/ml) and MBV (5 µg/ml) neutralized these increases. (d,f) Similar to microglia, LPS/IFNγ increased IL-1β, IL-6, and TNF-α secretion from unprimed astrocytes. Media conditioned by primed microglia also increased IL-1β, IL-6, and TNF-α but UBM-ECM and MBV neutralized these increases. (g,h) Tables show the mean ± SEM for the (g) Microglia and (h) Astrocyte data in graphs (a–f) Data represent triplicates from three independent experiments. (i–k) RGCs were cultured in media conditioned by unprimed or primed astrocytes. (i) After 3 DIV, RGCs treated with either LPS/IFNγ in unprimed media or primed media alone showed 100% RGC death. RGC cultures treated with unprimed media with MBV or primed media with UBM-ECM or MBV had increased numbers of viable cells compared to control. (h,i) Representative live (green) and dead (red) images of RGCs in primed astrocyte media (j) without or (k) with MBV. Data represent n > 300 neurons analyzed from 3 independent experiments normalized to viability in unconditioned media. Error bars indicate the SEM. One-way ANOVA determined significance between groups, *p < 0.05, and compared to unconditioned media, #p < 0.01.
