Table 1 Parameters extracted from FRAP curves. Cells seeded onto micropatterned and homogeneously coated OKT3 surfaces were fit individually with Eq. 1 and Eq. 2, respectively.

From: A micropatterning platform for quantifying interaction kinetics between the T cell receptor and an intracellular binding protein

T

min after seeding

 

τ1(s)

τ2/τ(s)

f m

b

c

N

22 °C

5–45

patterned OKT3

—

14.6 ± 1.0

0.78 ± 0.04

0.12 ± 0.02

—

34

coated OKT3

1.0 ± 0.1

29.7 ± 1.9

0.78 ± 0.04

0.10 ± 0.02

0.34 ± 0.02

28

37 °C

5–15

patterned OKT3

—

6.7 ± 1.1

0.68 ± 0.03

0.07 ± 0.01

—

8

coated OKT3

1.6 ± 0.3

15.6 ± 3.7

0.76 ± 0.04

0.21 ± 0.05

0.33 ± 0.07

7

25–45

patterned OKT3

—

4.7 ± 0.90

0.57 ± 0.04

0.05 ± 0.01

—

8

coated OKT3

1.2 ± 0.2

20.7 ± 5.3

0.57 ± 0.05

0.17 ± 0.04

0.43 ± 0.05

8

  1. Mean fit values ± SEM are listed for τ1, τ2, fm, b and c. For cells measured at 22 °C, all cells shown in Fig. 5 were included. For data recorded at 37 °C, cells were grouped according to the timepoints of their measurement (5–15 minutes and 25–45 minutes after seeding). N indicates the number of cells in the time group. Data were recorded in at least 2 independent experiments.
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