Figure 3
From: SOX6 blocks the proliferation of BCR-ABL1+ and JAK2V617F+ leukemic cells
SOX6 induces erythroid genes at the expense of genes affiliated with other lineage programs. (a) Expression level of marker genes upon SOX6 transduction. Fold change variation in different lineage-specific genes (erythroid, megakaryocytic, lymphoid) was assessed by RT-qPCR (n ≥ 3). In the erythroleukemic HEL cells overexpressing SOX6 the enhanced erythroid differentiation was confirmed by cells staining. HBA: Hemoglobin α chain; HBG: Hemoglobin γ chain; ALAS-E: Aminolevulinate Synthase, Erythroid; GPIIb: Glycoprotein IIB; RUNX1: Runt-related transcription factor 1; FLI1: Friend leukemia integration 1 transcription factor; MPL: thrombopoietin receptor; PAX5: Paired Box 5; EBF1: Early B Cell Factor 1; BLNK: B Cell Linker. (b) Cells distribution across the different cell cycle phases analyzed by Propidium Iodide staining (PI). Dark gray: G2/M phase; light gray: S phase; white: G0/G1 phase. Arrows indicate significant reduction of the percentage of cells in S-phase.
