Figure 4
MβCD-cholesterol treatment drastically increases SLO pore formation. (A) FreeStyle293-F cells transiently transfected with plasmids encoding ABCA1-GFP were treated with (right) or without (left) MβCD–cholesterol complex just before flow cytometry analysis. Cells with fluorescence intensity greater than 50,000 were defined as ABCA1-GFP–positive, and others as ABCA1-GFP–negative. The median value of Alexa Fluor 647 fluorescence intensity of each population is shown. (B) HEK293 cells transiently transfected with plasmids encoding ABCA1-GFP were treated with (right) or without (left) MβCD–cholesterol complex just before the SLO treatment (original images are shown in Supplementary Fig. 1). (C) DAPI-stained and -unstained cells in GFP-positive cells were counted, and proportions were calculated. Average values are shown with S.E. For each sample, images were acquired at five positions in the dish. **P < 0.001 vs. cells not treated with MβCD–cholesterol complex.
