Table 1 Effect of CHAPS and PS80 on the attachment of low and high biofilm formers to a plastic substrate.

From: Inhibitory effects of polysorbate 80 on MRSA biofilm formed on different substrates including dermal tissue

bacteria

cultured at

control

bacteria × 103/cm2 plastic chips

0.5% CHAPS

reduction (%)

0.5% PS80

reduction (%)

T109

0 °C

49.0 ± 5.4

30.7 ± 10.8

37

15.7 ± 4.1**

68

37 °C

215.8 ± 24.5

114.2 ± 17.1*

47

21.6 ± 3.5**

90

ATCC BAA-2856

0 °C

360.1 ± 31.2

223.6 ± 50.3*

38

15.2 ± 3.8**

96

37 °C

710.6 ± 51.2

142.8 ± 7.3**

80

23.4 ± 6.1**

97

  1. T109, a low biofilm-forming MRSA, and ATCC BAA-2856, a high biofilm-forming MRSA, were used. Plastic chips (1 × 1 cm pieces of OHP sheet) were immersed in confluent bacterial solutions containing 0.5% PS80 or 0.5% CHAPS, both of which exerted biofilm inhibitory effects as shown in Fig. 2. They were incubated at 0 °C or 37 °C for 1 h. After incubation, the bacteria adhered on the chip were visualized using FITC-labeled S. aureus antibody, and their number was counted by a morphometrical method as described in the Materials and Methods. Data are presented as number of bacteria/cm2 area, mean ± SE, *p < 0.05 and **p < 0.01 vs. control in each temperature. Sample number/group ≥8, duplicated.
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