Figure 3 | Scientific Reports

Figure 3

From: Dental pulp cell-derived powerful inducer of TNF-α comprises PKR containing stress granule rich microvesicles

Figure 3

DPIT also stimulates undifferentiated THP-1 cells. Floating undifferentiated THP-1 (unTHP-1) cells were stimulated with DP-1 sup or PMA for indicated time periods and cell attachment was visualized with phase-contrast microscopy after non-attached cells are washed out (a). DP-1 sup promoted cell attachment to the culture plate as PMA. Simultaneously, total RNAs are also collected to see the TNF-α gene expression in unTHP-1 cells stimulated with DP-1 sup or PMA (b). Both DP-1 sup and PMA promoted TNF-α gene expression.

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