Figure 4
THP-1 cell activation by DPIT is independent of PKC activation. (a) Floating unTHP-1 cells were stimulated for 3 hrs with DP-1 sup or PMA with a PKC inhibitor (GF109203X: 10 μM) and cell attachment was visualized with phase-contrast microscopy. (b) Simultaneously, total RNAs are collected to see TNF-α gene expression (b). Although PKC inhibitor markedly suppressed unTNP-1 cell attachment to the culture plate and TNF-α gene expression in unTHP-1 cells in the presence of PMA, no inhibitory effect was observed when the cells were stimulated DP-1 sup. Data represents means ± SD of at least two independent experiments with identical results. Statistical analyses were performed by Student’s t-test. **p < 0.01, ***p < 0.001, significantly difference versus cells untreated with PKC inhibitor.
