Figure 9
Uptake of dental pulp cell-derived MVs by recipient dTHP-1 cells. (a,b) DP-1-MVs uptake triggers TNF-α production in dTHP-1 cells. PKH67-labeled DP-1-MVs are incorporated by dTHP-1 cells (a), while cellular uptake (a) and TNF-α production (b) are suppressed by pre-treatment of dTHP-1 cells with nystatin, but not MBCD (a,b). DP-1 cell-derived MVs are labeled with PKH67 and cellular uptake by dTHP-1 cells were observed by fluorescent microscopy as described in “Methods”. The cells were pretreated with or without nystatin or MBCD to suppress lipid raft mediated endocytosis or to achieve cholesterol depletion from culture medium. Data represent means ± SD of at least two independent experiments with identical results. Statistical analyses were performed by one-way ANOVA, followed by Dunnett’s test (b). *p < 0.05, **p < 0.01, ***p < 0.001. significant difference versus dTHP-1 cells stimulated with MVs in the absence of nystatin or MBCD (b).
