Figure 2 | Scientific Reports

Figure 2

From: Vector uncoating limits adeno-associated viral vector-mediated transduction of human dendritic cells and vector immunogenicity

Figure 2

Selection of AAV capsid variants on iDC. (A) Quantification of NGS results. Variants that accumulated after two rounds of AAV2-based peptide library selection on iDC from different donors (Supplementary Fig. S3) were subjected to NGS using cap-specific primers to identify peptide sequences that mediated cell infection. Shown are sequences of peptide inserts present with amounts >1.5%. PTRLLP is excluded owning to its smaller size. (B) Phylogenic analysis of top AAV variants. Amino acid sequences of peptide inserts were aligned. A phylogenic tree was constructed using the maximum likelihood method. Selected variants were thereby classed into two main families. Numbers indicate bootstrap values.

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